Geodesic
Marcatura retrograda selettiva con D-$\left[^{3}H\right]$ Aspartato dei neuroni delle proiezioni cortico-corticali destinate all'area SI
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 81 (1987) no. 4, pp. 433-438 
Barbaresi, Paolo; Fabri, Mara; Conti, Fiorenzo; Manzoni, Tullio. Marcatura retrograda selettiva con D-$\left[^{3}H\right]$ Aspartato dei neuroni delle proiezioni cortico-corticali destinate all'area SI. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 81 (1987) no. 4, pp. 433-438. http://geodesic.mathdoc.fr/item/RLINA_1987_8_81_4_a10/
@article{RLINA_1987_8_81_4_a10,
     author = {Barbaresi, Paolo and Fabri, Mara and Conti, Fiorenzo and Manzoni, Tullio},
     title = {Marcatura retrograda selettiva con {D-}$\left[^{3}H\right]$ {Aspartato} dei neuroni delle proiezioni cortico-corticali destinate all'area {SI}},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {433--438},
     year = {1987},
     volume = {Ser. 8, 81},
     number = {4},
     language = {it},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1987_8_81_4_a10/}
}
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Voir la notice de l'article provenant de la source Biblioteca Digitale Italiana di Matematica

A solution of D-$\left[^{3}H\right]$Aspartate mixed with horseradish peroxidase (HRP) was injected in the trunk and forelimb representation zones of area SI. Neurones labelled with D-$\left[^{3}H\right]$Aspartate were absent from the thalamus ipsilateral to the injected areas, but numerous neurones retrogradely labelled with this radioactive marker were present in the ipsilateral area SII (SII-SI associative neurones) and in the contralateral area SI (SI-SI callosal neurones). In both areas, cells labelled with silver grains were topographically distributed in the same regions containing cells retrogradely labelled with HRP. Associative and callosal neurones retrogradely labelled with D-$\left[^{3}H\right]$Aspartate showed a laminar pattern similar to that of associative and callosal neurones labelled with HRP, with one exception. In the inner half of layer III, associative and callosal neurones labelled with D-$\left[^{3}H\right]$Aspartate were much less numerous than those labelled with HRP. The present results indicate that D-$\left[^{3}H\right]$Aspartate is a selective tracer for a high proportion of associative and callosal neurones of areas SI and SII. These labelled neurones therefore are likely to use Aspartate and/or Glutamate as neurotransmitter. The reasons by which cortico-cortical neurons of lower layer III were unlabelled remain unsolved. These neurones might utilize different chemical(s) as neurotransmitter(s) or, alternatively, they might represent a case of phalse negativity.

[1] Alloway K.D. and Burton H. (1985) - Homotypical ipsilateral cortical projections between somatosensory areas I and II in the cat. «Neuroscience», 14, 15-35.

[2] Baughman R.W. and Gilbert C.D. (1981) - Aspartate and glutamate as possible neurotransmitters in the visual cortex. «J. Neurosci.», 1, 427-439.

[3] Burton H., Mitchell G. and Brent D. (1982) - Second somatic sensory area in the cerebral cortex of cats: somatotopic organization and cytoarchitecture. «J. Comp. Neurol.», 210, 109-135.

[4] Caminiti R., Innocenti G.M. and Manzoni T. (1979) - Anatomical substrate of the callosai messages form SI and SII in the cat. «Exp. Brain Res.», 35, 295-314.

[5] Cuḗnod M., Bagnoli P., Beaudet A., Rustioni A., Wiklund L. and Streit P. (1982) - Transmitter-specific retrograde labeling of neurons. In: Chan-Palay V. and Palay S.L. (eds.), Cytochemical Methods in Neuroanatomy, Alan Liss, New York, pp. 17-44.

[6] Fisher B.O., Ottersen O.P. and Storm-Mathisen J. (1982) - Axonal transport of D-$\left[^{3}H\right]$aspartate in the claustrocortical projection. «Neuroscience (Suppl. 2)», S 69.

[7] Graham R.C.J. and Karnowsky M.J. (1966) - The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. «J. Histochem. Cytochem.», 14, 291-302.

[8] JONES E.G.(1985) - The Thalamus, XVII, 935, Pleunum Press, New York.

[9] Manzoni T., Barbaresi P., Bellardinelli E. and Caminiti R. (1980) - Callosal projections from the two body midlines. «Exp. Brain Res.», 39, 1-9.

[10] Manzoni T., Barbaresi P. and Fabri M. (1986a) - D-$\left[^{3}H\right]$Aspartate retrograde labelling of association neurones in area SI of the cat. «Neurosci. Lett.», 61, 175-180.

[11] Matute C. and Streit P. (1985) - Selective retrograde labeling with D-$\left[^{3}H\right]$aspartate in afferents to mammalian superior colliculus. «J. Comp. Neurol.», 241, 34-49.

[12] Mesulam M.M. (1978) - Tetramethylbenzidine for horseradish peroxidase neurohistochemistry: A non carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. «J. Histochem. Cytochem.», 26, 160-177.

[13] Ottersen O.P., Fisher B.O. and Storm-Mathisen J. (1983) - Retrograde transport of D-$\left[^{3}H\right]$Aspartate in thalamocortical neurones. «Neurosci. Lett.», 42, 19-24.

[14] Rustioni A. and Cuḗnod M. (1982) - Selective retrograde transport of D-aspartate in spinal interneurons and cortical neurons of rats. «Brain Res.», 236, 143-155.

[15] Rustioni A., Schmechel D.E., Spreafico R., Cheema S. and Cuenod M. (1983) - Excitatory and inhibitory amino acid putative neurotransmitters in the ventralis posterior complex: an autoradiographic and immunocytochemical study in rats and cats. In: G. MACCHI, A. RUSTIONI and R. SPREAFICO (eds.) Somatosensory Integration in the Thalamus, Elsevier, Amsterdam, pp. 365-383.

[16] Streit P. (1980) - Selective retrograde labeling indicating the transmitter of neuronal pathways. «J. Comp. Neurol.», 191, 429-463.

[17] Streit P. (1984) - Glutamate and aspartate as transmitter candidates for systems of the cerebral cortex. In: E.G. JONES and A. PETERS (eds.) Cerebral Cortex, Vol. 2, Functional properties of cortical cells, Plenum Press, New York, pp. 119-143.