Geodesic
Nanomodified Bacteria: Detection of Individual Cells Using Surface-Enhanced Raman Scattering
Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki, Kazanskii Gosudarstvennyi Universitet. Uchenye Zapiski. Seriya Fiziko-Matematichaskie Nauki, Tome 151 (2009) no. 1, pp. 82-89
Cet article a éte moissonné depuis la source Math-Net.Ru

Voir la notice du chapitre de livre

The layer-by-layer method of coating bacterial cells with polyelectrolytes and silver nanoparticles for improving the surface-enhanced Raman scattering (SERS) spectra is reported. First, the bacteria cell wall is coated with poly(allylamine) hydrochloride (PAH) and/or poly(styrene) sulfonate (PSS) and then with citrate reduced silver nanoparticles. In order to increase the stability of the coating, other layers of PAH/PSS are formed on the surface. The SEM and AFM images indicate isolated nanoparticles and aggregates of nanoparticles on the bacterial wall. The coating of bacterial cells with silver nanoparticles not only serves for their preparation for SERS measurement but also helps to visualize the coated bacterial cells under the ordinary whitelight microscope objective due to efficient light scattering properties of silver nanoparticles. The two bacteria that differ in shape and cell wall biochemical structure, Escherichia coli and Staphylococcus cohnii, Gram-negative and Gram-positive respectively, are used as models. Reproducible SERS spectra were obtained from single and aggregated silver nanoparticle-coated cells. These spectra can provide valuable information about chemical structure of bacterial cell wall. Preliminary results reveal that the approach could be used for single bacterial cell identification. Key words: layer-by-layer, polyelectrolyte nanofilms, silver nanoparticles, single cell, surface-enhanced Raman scattering.
Keywords: layer-by-layer method, polyelectrolyte nanofilms, single cell, surface-enhanced Raman scattering.
Mots-clés : silver nanoparticles 
@article{UZKU_2009_151_1_a10,
     author = {A. I. Zamaleeva and M. Kahraman and M. Culha and R. F. Fakhrullin},
     title = {Nanomodified {Bacteria:} {Detection} of {Individual} {Cells} {Using} {Surface-Enhanced} {Raman} {Scattering}},
     journal = {U\v{c}\"enye zapiski Kazanskogo universiteta. Seri\^a Fiziko-matemati\v{c}eskie nauki},
     pages = {82--89},
     year = {2009},
     volume = {151},
     number = {1},
     language = {ru},
     url = {http://geodesic.mathdoc.fr/item/UZKU_2009_151_1_a10/}
}
TY  - JOUR
AU  - A. I. Zamaleeva
AU  - M. Kahraman
AU  - M. Culha
AU  - R. F. Fakhrullin
TI  - Nanomodified Bacteria: Detection of Individual Cells Using Surface-Enhanced Raman Scattering
JO  - Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki
PY  - 2009
SP  - 82
EP  - 89
VL  - 151
IS  - 1
UR  - http://geodesic.mathdoc.fr/item/UZKU_2009_151_1_a10/
LA  - ru
ID  - UZKU_2009_151_1_a10
ER  - 
%0 Journal Article
%A A. I. Zamaleeva
%A M. Kahraman
%A M. Culha
%A R. F. Fakhrullin
%T Nanomodified Bacteria: Detection of Individual Cells Using Surface-Enhanced Raman Scattering
%J Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki
%D 2009
%P 82-89
%V 151
%N 1
%U http://geodesic.mathdoc.fr/item/UZKU_2009_151_1_a10/
%G ru
%F UZKU_2009_151_1_a10
A. I. Zamaleeva; M. Kahraman; M. Culha; R. F. Fakhrullin. Nanomodified Bacteria: Detection of Individual Cells Using Surface-Enhanced Raman Scattering. Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki, Kazanskii Gosudarstvennyi Universitet. Uchenye Zapiski. Seriya Fiziko-Matematichaskie Nauki, Tome 151 (2009) no. 1, pp. 82-89. http://geodesic.mathdoc.fr/item/UZKU_2009_151_1_a10/

[1] Smith C. J., Osborn A. M., “Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology”, FEMS Microbiol Ecol., 67:1 (2009), 6–20 | DOI

[2] Naumann D., Helm D., Labischinski H., Giesbrecht P., “The characterization of microorganisms by Fourier transform infrared spectroscopy (FT-IR)”, Modern Techniques for Rapid Microbiological Analysis, 1991, 133–141

[3] Puppels G. J., De Mul F. F., Otto C., Greve J., Robert-Nicoud M., Arndt-Jovin D. J., “Studying single living cells and chromosomes by confocal Raman microspectroscopy”, Nature (London), 347 (1990), 301–303 | DOI

[4] Otto A., “Investigations of electrode surfaces in acetonitrile solutions using surface-enhanced Raman spectroscopy”, Light Scattering in Solids, 4 (1984), 289–296

[5] Premasiri W. R., Moir D. T., Klempner M. S., Krieger N., Jones G., Ziegler L. D., “Characterization of the Surface Enhanced Raman Scattering (SERS) of Bacteria”, J. Phys. Chem., 109 (2005), 312–320 | DOI

[6] Chang R. K., Furtak T. E., Surface Enhanced Raman Scattering, Plenum Press, New York–London, 1982, 432 pp.

[7] Laucks M. L., Sengupta A., Junge K., Davis E. J., Swanson B. D., “Comparison of Psychro-active Arctic Marine Bacteria and Common Mesophillic Bacteria Using Surface-Enhanced Raman Spectroscopy”, Appl. Spectrosc., 59 (2005), 1222–1228 | DOI

[8] Kahraman M., Yazici M. M., Şahin F., Bayrak Ö. F., Çulha M., “Reproducible Surface-Enhanced Raman Scattering Spectra of Bacteria on Aggregated Silver Nanoparticles”, Appl. Spectrosc., 61 (2007), 479–485 | DOI

[9] Taton T. A., Mirkin C. A., Letsinger R. L., “Scanometric DNA Array Detection with Nanoparticle Probes”, Science, 289 (2000), 1757–1760 | DOI

[10] Jarvis R. M., Brooker A., Goodacre R., “Rapid analysis of microbiological systems using SERS”, Anal. Chem., 76 (2004), 5198–5202 | DOI

[11] Arida A. I., Al-Tabakha M. M., “Encapsulation of ketoprofen for controlled drug release”, Eur. J. Pharm. Biopharm., 66:1 (2007), 48–54 | DOI

[12] Correa-Duarte M. A., Kosiorek A., Kandulski W., Giersig M., Liz-Marzan L. M., “Layer-by-Layer Assembly of Multiwall Carbon Nanotubes on Spherical Colloids”, Chem. Mater., 17:12 (2005), 3268–3272 | DOI

[13] G. Decher, J. B. Schlenoff (eds.), Multilayer Thin Films, Wiley-VCH Verlag GmbH Co., 2002, 535 pp.

[14] Diaspro A., Silvano D., Krol S., Cavalleri O., Gliozzi A., “Single living cell encapsulation in nano-organized polyelectrolyte shells”, Langmuir, 18 (2002), 5047–5050 | DOI

[15] Sukhorukov G. B., Donath E., Lichtenfeld H., Knippel E., Knippel M., Budde A., Möhwald H., “Layer-by-layer Self Assembly of Polyelectrolytes on Colloidal Particles”, Colloid Surface A, 137:1–3 (1998), 253–266 | DOI

[16] Zeiri L., Bronk B., Shabtai V. Y. et al., “Surface-Enhanced Raman Spectroscopy as a Tool for Probing Specific Biochemical Components in Bacteria”, Appl. Spectrosc., 58 (2004), 33–40 | DOI

[17] Kahraman M., Muge Y. M., Fikrettin Süahin, Culha M., “Convective Assembly of Bacteria for Surface-Enhanced Raman Scattering”, Langmuir, 24 (2008), 894–901 | DOI

[18] Sengupta A., Mujacic M., Davis E., “Detection of bacteria by surface-enhanced Raman spectroscopy”, J. Anal. Bioanal. Chem., 386 (2006), 1379–1388 | DOI