Geodesic
Comparative analysis of the effects of platinum compounds on thermostability of DNA
Proceedings of the Yerevan State University. Physical and mathematical sciences, no. 3 (2011), pp. 52-57

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The thermal denaturation profiles of Calf Thymus DNA in the presence of binuclear compounds of bivalent platinum $\mathrm{(cis-[\{Pt(NH_3)_2Cl\}_2Pyr]Cl_2)}$ were studied by absorption spectrophotometry and differential adiabatic scanning microcalorimetry. A comparison with an active antitumor drug cis-diamminedichloroplatinum (cis-DDP) was made. Experimental data witness that small concentrations $(r0.3)$ of binuclear platinum compound stabilized the structure of Calf Thymus DNA stronger than cis-DDP. Both the complexes tend to refold the denaturated DNA. It is rather interesting that in case of $10^{-6}M$ concentration of binuclear platinum compound $(r=0.03)$ the denaturated DNA is refolded by $77.62\%$, whereas under the same condition cis-DDP leads to $49.01\%$ refolding of denaturated DNA. Such a high percent of renaturation was probably the result of interstrand cross-links formation.
Keywords: DNA, binuclear compound of bivalent platinum, DNA melting, interstrand cross-link.
Mots-clés : cis-DDP, renaturation 
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     title = {Comparative analysis of the effects of platinum compounds on thermostability of {DNA}},
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I. E. Grigoryan; Y. B. Dalyan; S. G. Arutyunyan. Comparative analysis of the effects of platinum compounds on thermostability of DNA. Proceedings of the Yerevan State University. Physical and mathematical sciences, no. 3 (2011), pp. 52-57. http://geodesic.mathdoc.fr/item/UZERU_2011_3_a7/