Geodesic
Catalytic properties of stem bromelain, ficin and papain in the acidic pH region. A comparative pre-steady-state and steady-state study
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 4, pp. 797-804

Voir la notice de l'article provenant de la source Biblioteca Digitale Italiana di Matematica

Pre-steady-state and steady-state kinetics for the hydrolysis of p-nitrophenyl esters of N-$\alpha$-carbobenzoxy (-L-) amino acids catalyzed by stem bromelain (from Ananas sativus; EC 3.4.22.4), ficin (from Ficus glabrata; EC 3.4.22.3) and papain (from Carica papaya; EC 3.4.22.2) have been determined between pH 2.5 and 6.0 (I = 0.1M) at 21±0.5°C. The different kinetic behaviour of these cysteine proteinases, especially in the pH dependence of the deacylation step, has been related to the nature of the amino acid residue present at position 158. In fact, differences in the intensity of the electrostatic field contributed by the 158 side chain may affect the acid-base equilibrium of the Cys25-His159 catalytic diad, and thus the enzyme activity. 
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     author = {Ascenzi, Paolo and Aducci, Patrizia and Amiconi, Gino and Menegatti, Enea and Guarneri, Mario and Ballio, Alessandro},
     title = {Catalytic properties of stem bromelain, ficin and papain in the acidic {pH} region. {A} comparative pre-steady-state and steady-state study},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {797--804},
     publisher = {mathdoc},
     volume = {Ser. 8, 82},
     number = {4},
     year = {1988},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_4_a18/}
}
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%0 Journal Article
%A Ascenzi, Paolo
%A Aducci, Patrizia
%A Amiconi, Gino
%A Menegatti, Enea
%A Guarneri, Mario
%A Ballio, Alessandro
%T Catalytic properties of stem bromelain, ficin and papain in the acidic pH region. A comparative pre-steady-state and steady-state study
%J Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali
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Ascenzi, Paolo; Aducci, Patrizia; Amiconi, Gino; Menegatti, Enea; Guarneri, Mario; Ballio, Alessandro. Catalytic properties of stem bromelain, ficin and papain in the acidic pH region. A comparative pre-steady-state and steady-state study. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 4, pp. 797-804. http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_4_a18/