Possible role of diacylglycerol second messengers in the control of morphogenesis in Hydra attenuata

Venturini, Giorgio ; Gioberti, Marianna

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 3, pp. 571-576.

Abstract (VO)

The effects induced by tumor promoters phorbol esters (TPA) on head regeneration and on development of cell aggregates were studied in Hydra attenuata. TPA inhibits head regeneration and often induces the appearance of abnormal body shapes. The development of cellular aggregates is severely alterated by TPA, and treated aggregates usually do not differentiate organized structures. In TPA treated aggregates the nerve cell number is dramatically reduced with respect to control aggregates. The results suggest the involvement of membrane phosphoinositides in the transduction of signals controlling the proliferation and differentiation of interstitial cells.

Riassunto

Sono stati studiati gli effetti indotti dal trattamento con esteri del forbolo promotori tumorali (TPA) sulla rigenerazione della testa e sullo sviluppo di aggregati ottenuti da cellule isolate in Hydra attenuata. Il TPA inibisce la rigenerazione della testa di animali amputati e induce spesso la comparsa di forme corporee anomale. Lo sviluppo degli aggregati cellulari risulta in presenza di TPA fortemente alterato, non raggiungendosi in genere la formazione di strutture organizzate. Negli aggregati risulta gravemente alterato il numero delle cellule nervose. I risultati vengono considerati a favore della implicazione dei fosfoinositidi di membrana nella trasduzione di segnali attivi sulla proliferazione e sul differenziamento delle cellule interstiziali.

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