GnRH-like immunoreactive substances in some différent tissues of the lizard, Podarcis s. sicula Raf, during the sexual cycle
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 3, pp. 561-569.

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The distribution of immunoreactive GnRH-like substances in various tissues of the lizard (Podarcis s. sicula) in relation to different phases of the sexual cycle are described. GnRH-like substances in the hypothalamic extracts decrease during the reproductive period. In the extrahypothalamic tissue, the levels of immunoreactive GnRH-like substances are highest in early spring and summer, and decrease in autumn, winter and late spring. In the other tissues (gonads, liver and small intestine), a more constant immunoreactivity has been observed. All tissue extracts stimulate the release of luteininzing hormone (LH) from chicken anterior pituitary cells. In testis this phenomenon is more evident in early spring and summer; in the other tissues, two peaks of activity can be detected, in spring and in autumn. These findings suggest that GnRH-like substances could be involved in the regulation of various aspects of reproductive processes in the lacertid Podarcis s. sicula Raf.
É stata determinata la presenza e la concentrazione di sostanze immunologicamente simili al GnRH in vari organi del lacertide Podarcis s. sicula, utilizzando tre anticorpi specifici per tratti diversi di questo peptide. Negli estratti di ipotalamo e di encefalo (privo di ipotalamo) i risultati delle reazioni immunologiche indicano la presenza di tali sostanze che vanno incontro a definire variazioni di concentrazione nel corso del ciclo riproduttivo (nell'ipotalamo diminuiscono in primavera; nelle regioni extraipotalamiche dell'encefalo sono più elevate all'inizio della primavera ed in estate). Negli altri organi esaminati (testicolo, ovario, fegato ed intestino) sono state evidenziate, essenzialmente, delle componenti che potrebbero avere sequenze di amminoacidi in parte simili a quelle del GnRH; esse, tuttavia, non mostrano delle chiare modificazioni nel corso dell'anno. Gli estratti di tutti gli organi esaminati posseggono la capacità di indurre in vitro il rilascio di LH dall'ipofisi di pollo. Tale proprietà risulta però piuttosto variabile nei diversi periodi dell'anno e spesso non in accordo con i dati ottenuti con le indagini immunologiche.
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     title = {GnRH-like immunoreactive substances in some diff\'erent tissues of the lizard, {Podarcis} s. sicula {Raf,} during the sexual cycle},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
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Ciarcia, Gaetano; Botte, Virgilio. GnRH-like immunoreactive substances in some différent tissues of the lizard, Podarcis s. sicula Raf, during the sexual cycle. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 3, pp. 561-569. http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_3_a20/

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