Geodesic
Alanine transport in purified plasma membrane vesicles from ascites hepatoma (Yoshida AH-130) cells in stationary phase of the in vivo growth
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 361-369 
Leonardi, Maria Giovanna; Cislaghi, Elena; Bonfanti, Patrizia; Comolli, Roberto; Giordana, Barbara. Alanine transport in purified plasma membrane vesicles from ascites hepatoma (Yoshida AH-130) cells in stationary phase of the in vivo growth. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 361-369. http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a18/
@article{RLINA_1988_8_82_2_a18,
     author = {Leonardi, Maria Giovanna and Cislaghi, Elena and Bonfanti, Patrizia and Comolli, Roberto and Giordana, Barbara},
     title = {Alanine transport in purified plasma membrane vesicles from ascites hepatoma {(Yoshida} {AH-130)} cells in stationary phase of the in vivo growth},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {361--369},
     year = {1988},
     volume = {Ser. 8, 82},
     number = {2},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a18/}
}
TY  - JOUR
AU  - Leonardi, Maria Giovanna
AU  - Cislaghi, Elena
AU  - Bonfanti, Patrizia
AU  - Comolli, Roberto
AU  - Giordana, Barbara
TI  - Alanine transport in purified plasma membrane vesicles from ascites hepatoma (Yoshida AH-130) cells in stationary phase of the in vivo growth
JO  - Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali
PY  - 1988
SP  - 361
EP  - 369
VL  - 82
IS  - 2
UR  - http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a18/
LA  - en
ID  - RLINA_1988_8_82_2_a18
ER  - 
%0 Journal Article
%A Leonardi, Maria Giovanna
%A Cislaghi, Elena
%A Bonfanti, Patrizia
%A Comolli, Roberto
%A Giordana, Barbara
%T Alanine transport in purified plasma membrane vesicles from ascites hepatoma (Yoshida AH-130) cells in stationary phase of the in vivo growth
%J Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali
%D 1988
%P 361-369
%V 82
%N 2
%U http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a18/
%G en
%F RLINA_1988_8_82_2_a18

Voir la notice de l'article provenant de la source Biblioteca Digitale Italiana di Matematica

An increased amino acid uptake occurs in neoplastic cells. In order to discriminate between cell metabolic effects and/or membrane changes, the transport of L-alanine has been studied in the present work using plasma membrane vesicles isolated from a highly undifferentiated ascites hepatoma cell line (Yoshida AH 130). Contrary to the data previously obtained with normal hepatocytes, in which alanine transport occurs via two different transport systems (A and ASC), kinetic studies with hepatoma membrane vesicles showed the presence of a unique component. However, the presence of two transport systems cannot be excluded in this hepatoma cell line, since the technique employed can only barely detect transport systems with similar kinetic constants.

[1] M.J. Weper (1973) - Hexose transport in normal and Rous sarcoma virus-transformed cells. «J. Biol. Chem.», 248, 2978-2983.

[2] M. Hatanaka (1974) - Transport of sugars in tumor cell membranes. «Biochim. Biophys. Acta.», 355, 77-104.

[3] R.F. Kletzien and J.F. Perdue (1974) - Sugar transport in chick embryo fibroblasts. II. Alteration in transport following transformation by a temperature sensitive mutant of the Rous sarcoma virus. «J. Biol. Chem.», 249, 3375-3382.

[4] D.O. Foster and A.B. Pardee (1969) - Transport of amino acid by confluent and non-confluent 3T3 and Polyoma virus-transformed 3T3 cells growing on glass cover slips. «J. Biol. Chem.», 144, 2675-2681.

[5] M. Inbar, H. Ben-Bassat and L. Sachs (1971) - Localization of amino acid and carbohydrate transport sites in the surface membrane of normal and transformed mammalian cells. «J. Membrane Biol.», 6, 195-205.

[6] K.J. Hisselbacher (1972) - Increased uptake of amino acid and 2-deoxy-D-glucose by virus transformed cells in culture. «Proc. Natn. Acad. Sci. USA», 69, 585-589.

[7] S.J. Friedman and P. Skehan (1981) - Malignancy and the cell surface. In: The transformed cell (I.L. Cameron and T.B. Pool, eds.), Academic Press, New York.

[8] R. Comolli, A. Casale and D. Mariotti (1984) - Amiloride and glucose effects on the intracellular pH of Yoshida rat ascites hepatoma AH-130 cells grown in vivo. «Cell Biol. Int. Rep.», 8, 297-307.

[9] E.F. Boumendil—Podevin and R.A. Podevin (1983) - Isolation of basolateral and brush-border membranes form rabbit kidney cortex. Vesicle integrity and membrane sidedness of the basolateral fraction. «Biochim. Biophys. Acta», 735, 86-94.

[10] W. Schoner, C. Von Ilberg, R. Kramer and W. Schubert (1967) - On the mechanism of $Na^{+}$ and $K^{+}$-stimulated hydrolysis of adenosine triphosphate. «Eur. J. Biochem.», 1, 334-345.

[11] R.M. Michell and J.N. Hawthone (1965) - The site of diphosphoinositine synthesis in rat liver. «Biochem. Biophys. Res. Comm.», 21, 333-338.

[12] B.S.S. Masters, C.H. Williams Jr and H. Kamin (1967) - The preparation and properties of microsomal TPNH-cytochrome c reductase from pig liver. «Methods in Enzymology», X, 565-573.

[13] L. Smith (1955) - Spectrophotometric assay of cytochrome c oxidase. In: «Methods of Biochemical analysis» vol. 2, Glick D. Ed. pp. 427-437. Interscience Pub., New York.

[14] M.M. Bradford (1976) - A rapid and sensitive method for the quantitation of protein utilizing the principle of dye-protein binding. «Anal. Biochem.», 72, 248-254.

[15] M.G. Leonardi, R. Comolli and B. Giordana (1986) - Sodium-dependent amino acid transport in plasma membrane vesicles from rat liver. «Atti Accad. Naz. Lincei», 80, 313-322.

[16] M. Samson and M. Fehlmann (1982) - Plasma membrane vesicles from isolated hepatocytes retain the increase of amino acid transport by dibutyryl cyclic AMP in intact cells. «Biochim. Biophys. Acta», 687, 35-41.

[17] H.J. Sips, J.M.M. Van Amelsvoort and K. Van Dam (1980) - Amino acid transport in plasma membrane vesicles from rat liver. «Eur. J. Biochem.», 105, 216-224.

[18] J.W. Edmonson, L. Lumeng and T.-K. Li (1979) - Comparative studies of alanine and $\alpha$-aminoisobutyric acid uptake by freshly isolated rat liver cells. «J. Biol. Chem.», 254, 1653-1658.

[19] M.S. Kilberg, M.E. Hanlogten and H.N. Christensen (1981) - Characteristics of system ASC for transport of neutral amino acids in isolated rat hepatocyte. «J. Biol. Chem.», 256, 3304-3312.

[20] L.O. Kristensen (1980) - Energization of alanine transport in isolated rat hepatocytes. «J. Biol. Chem.», 255, 5236-5243.

[21] D.C. Quilland, C.G. Todderud, D.S. Kelley and R.F. Kletzien (1982) - Sodium-gradient-stimulated transport of L-alanine by plasma-membrane vesicles isolated from liver parenchymal cells of fed and starved rats. «Biochem. J.», 208, 685-693.

[22] M.A. Shotwell and D.L. Oxender (1983) - The regulation of neutral amino acid transport by amino acid availability in animal cells. «TIBS», 36, 314-316.

[23] M.E. Handlogten, R. Garcia-Canero, K.T. Lancaster and H.N. Christensen (1981) - Surprising differences in substrate selectivity and other properties of system A and ASC between rat hepatocytes and the hepatoma cell line HTC. «J. Biol. Chem.», 256, 7905-7909.

[24] I.H. Segal (1975) - Enzyme kinetics. John Wiley and Sons, New York.