Alanine transport in purified plasma membrane vesicles from ascites hepatoma (Yoshida AH-130) cells in stationary phase of the in vivo growth

Leonardi, Maria Giovanna   ; Cislaghi, Elena   ; Bonfanti, Patrizia   ; Comolli, Roberto   ; Giordana, Barbara

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 361-369

Résumé

An increased amino acid uptake occurs in neoplastic cells. In order to discriminate between cell metabolic effects and/or membrane changes, the transport of L-alanine has been studied in the present work using plasma membrane vesicles isolated from a highly undifferentiated ascites hepatoma cell line (Yoshida AH 130). Contrary to the data previously obtained with normal hepatocytes, in which alanine transport occurs via two different transport systems (A and ASC), kinetic studies with hepatoma membrane vesicles showed the presence of a unique component. However, the presence of two transport systems cannot be excluded in this hepatoma cell line, since the technique employed can only barely detect transport systems with similar kinetic constants.

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