Geodesic
Separation and characterisation of the lens proteins of the amphibian Rana esculenta
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 353-359 Cet article a éte moissonné depuis la source Biblioteca Digitale Italiana di Matematica

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In the present work the soluble lens proteins of Rana esculenta were studied by ion exchange chromatography, polyacrylamide gel electrophoresis and by immunoelectrophoresis. The column chromatography of total lens proteins, carried out with $NaH_{2}PO_{4}-Na_{2}HPO_{4}-NaCl$ buffer system revealed the presence of three main fractions. The first eluted, and predominant fraction, corresponds to gamma crystallins. Alpha and beta fractions follow as abundance. Further chromatography with a Tris-HCl buffer system of the isolated and concentrated gamma crystallins fraction showed six distinguishable components: this result was confirmed by acrylamide gel electrophoresis. Immunoelectrophoretic analysis of total lens proteins demonstrated a complex pattern with the presence of a minor, fast component that can be identified with the pre-alpha crystallins. 
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     author = {Bosco, Luigi and Venturini, Giorgio},
     title = {Separation and characterisation of the lens proteins of the amphibian {Rana} esculenta},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {353--359},
     year = {1988},
     volume = {Ser. 8, 82},
     number = {2},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a17/}
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Bosco, Luigi; Venturini, Giorgio. Separation and characterisation of the lens proteins of the amphibian Rana esculenta. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 353-359. http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a17/

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