Geodesic
Separation and characterisation of the lens proteins of the amphibian Rana esculenta
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 353-359.

Voir la notice de l'article provenant de la source Biblioteca Digitale Italiana di Matematica

In the present work the soluble lens proteins of Rana esculenta were studied by ion exchange chromatography, polyacrylamide gel electrophoresis and by immunoelectrophoresis. The column chromatography of total lens proteins, carried out with $NaH_{2}PO_{4}-Na_{2}HPO_{4}-NaCl$ buffer system revealed the presence of three main fractions. The first eluted, and predominant fraction, corresponds to gamma crystallins. Alpha and beta fractions follow as abundance. Further chromatography with a Tris-HCl buffer system of the isolated and concentrated gamma crystallins fraction showed six distinguishable components: this result was confirmed by acrylamide gel electrophoresis. Immunoelectrophoretic analysis of total lens proteins demonstrated a complex pattern with the presence of a minor, fast component that can be identified with the pre-alpha crystallins.
Nella presente ricerca sono state studiate le proteine solubili della lente di adulti di Rana esculenta mediante cromatografia a scambio ionico su colonna, elettroforesi su gel di poliacrilamide ed analisi immunoelettroforetica. La cromatografia su colonna delle proteine totali della lente, effettuata con il sistema tampone $NaH_{2}PO_{4}-Na_{2}HPO_{4}-NaCl$, ha rivelato la presenza di sei frazioni. La prima frazione eluita corrisponde alle $\gamma$ cristalline, e rappresenta la frazione predominante; seguono, in ordine di abbondanza, la frazione $\beta$ e la frazione ©. Una ulteriore cromatografia della frazione $\gamma$, isolata e concentrata, ha rivelato la presenza di sei distinte componenti, la cui presenza è confermata dall'analisi elettroforetica su gel di poliacrilamide. L'analisi immunoelettroforetica delle proteine totali ha evidenziato un quadro complesso con la presenza di una componente minore che può essere identificata come una frazione pre-$\alpha$. 
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     author = {Bosco, Luigi and Venturini, Giorgio},
     title = {Separation and characterisation of the lens proteins of the amphibian {Rana} esculenta},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {353--359},
     publisher = {mathdoc},
     volume = {Ser. 8, 82},
     number = {2},
     year = {1988},
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     url = {http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a17/}
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Bosco, Luigi; Venturini, Giorgio. Separation and characterisation of the lens proteins of the amphibian Rana esculenta. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 82 (1988) no. 2, pp. 353-359. http://geodesic.mathdoc.fr/item/RLINA_1988_8_82_2_a17/

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