Geodesic
Distribution of phosphoglucomutase (PGM) isoelectrophoretic alleles temperature-resistant (tr) and temperature-sensitive (ts) in two laboratory populations of Aedes aegypti
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 81 (1987) no. 2, pp. 209-217

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Phosphoglucomutase phenotypes of 440 individuals from two laboratory populations of Aedes aegypti have been determined by combining standard electrophoresis with a heat denaturation technique. This study has revealed the existence of two different PGM 0.90 isozymes, which were termed thermorésistant (tr) and thermosensitive (ts) depending on whether after heat treatment they were electrophoretically detectable or not. The analysis of the progeny phenotypes obtained in informative crosses involving both electrophoretic and thermostability variants showed a segregation pattern which conformed very closely to the Mendelian expectations on the hypothesis that the observed differences in heat sensitivity reside at the Pgm structural locus. Therefore, two different $Pgm^{0.90}$ alleles were identified: $Pgm^{0.90.tr}$ and $Pgm^{0.90.ts}$, whose products—isoelectrophoretic in standard conditions—can be distinguished at high temperature. The two samples examined could be well differentiated from each other for both the frequencies of the shared Pgm alleles and the presence or absence of particular Pgm alleles. 
@article{RLINA_1987_8_81_2_a8,
     author = {Cicchetti, Rosadele and Cancrini, Gabriella and Argentin, Gabriella and Scozzari, Rosaria},
     title = {Distribution of phosphoglucomutase {(PGM)} isoelectrophoretic alleles temperature-resistant (tr) and temperature-sensitive (ts) in two laboratory populations of {Aedes} aegypti},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {209--217},
     publisher = {mathdoc},
     volume = {Ser. 8, 81},
     number = {2},
     year = {1987},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1987_8_81_2_a8/}
}
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Cicchetti, Rosadele; Cancrini, Gabriella; Argentin, Gabriella; Scozzari, Rosaria. Distribution of phosphoglucomutase (PGM) isoelectrophoretic alleles temperature-resistant (tr) and temperature-sensitive (ts) in two laboratory populations of Aedes aegypti. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 81 (1987) no. 2, pp. 209-217. http://geodesic.mathdoc.fr/item/RLINA_1987_8_81_2_a8/