Isolamento di mutanti asporigeni di Bacillus thuringiensis ottenuti mediante bromuro di etidio
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 54 (1973) no. 2, pp. 293-298
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Acrystalliferous and asporigenous mutants of Bacillus thuringiensis were induced by ethidium bromide; the morphology of their colonies was altered. The high percentage of mutant colonies obtained suggests that mutations induced by ethidium bromide involve some genetic extrachromosomal determinants. The wild-type and the mutant strain DNAs were studied in the analytical centrifuge. The results showed that the DNA of B. thuringiensis has a G+C content of about 39% whereas no density difference exists between the sporeforming and the asporigenous bacteria.
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