Geodesic
Illumination system for sub-diffraction resolution microscopy
Vestnik Ûžno-Uralʹskogo gosudarstvennogo universiteta. Seriâ, Matematika, mehanika, fizika, Tome 14 (2022) no. 3, pp. 68-78

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Illumination scheme for superresolution microscopy is developed. The scheme accomplishes dark filed illumination with a laser light source including spatial coherence suppression. The scheme allows to observe nanoparticles with a size smaller than 50 nm. This is necessary to get higher resolution in the previously proposed method of superresolution microscopy (Near field Optical Random Microscopy - NORM). This method is based on real-time video processing of a nanoparticles Brownian motion those are located near the object surface. The method of vertical coordinate measurement is demonstrated. This method is based on astigmatic nanoparticle imaging. Three-dimensional distributions of suspended nanoparticles are obtained. Vertical resolution better than 200 nm and lateral resolution better than 100 nm are demonstrated.
Keywords: microscopy, nanoscopy, near-field microscopy, super-resolution, particle trajectory analysis, video processing.
Mots-clés : nanoparticles 
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     author = {S. A. Asselborn and E. S. Zatsepin and D. S. Isakov and A. M. Gerasimov and D. G. Pikhulya and Yu. V. Miklyaev},
     title = {Illumination system for sub-diffraction resolution microscopy},
     journal = {Vestnik \^U\v{z}no-Uralʹskogo gosudarstvennogo universiteta. Seri\^a, Matematika, mehanika, fizika},
     pages = {68--78},
     publisher = {mathdoc},
     volume = {14},
     number = {3},
     year = {2022},
     language = {ru},
     url = {http://geodesic.mathdoc.fr/item/VYURM_2022_14_3_a7/}
}
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%A E. S. Zatsepin
%A D. S. Isakov
%A A. M. Gerasimov
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%A Yu. V. Miklyaev
%T Illumination system for sub-diffraction resolution microscopy
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S. A. Asselborn; E. S. Zatsepin; D. S. Isakov; A. M. Gerasimov; D. G. Pikhulya; Yu. V. Miklyaev. Illumination system for sub-diffraction resolution microscopy. Vestnik Ûžno-Uralʹskogo gosudarstvennogo universiteta. Seriâ, Matematika, mehanika, fizika, Tome 14 (2022) no. 3, pp. 68-78. http://geodesic.mathdoc.fr/item/VYURM_2022_14_3_a7/