Spatial structure of HIV-enhancing heptapeptide Glu-Ile-Leu-Asn-His-Met-Lys in solution and in the complex with a model biological membrane

I. Z. Rakhmatullin   ; D. S. Blokhin   ; O. V. Aganova   ; A. R. Yulmetov   ; A. V. Filippov   ; A. V. Aganov   ; V. V. Klochkov

Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki, Uchenye Zapiski Kazanskogo Universiteta. Seriya Fiziko-Matematicheskie Nauki, Tome 154 (2012) no. 1, pp. 63-73

Résumé

The spatial structure of a synthetic fragment of SEVI – heptapeptide Glu-Ile-Leu-Asn-His-Met-Lys – in solution and in the complex with a model cell membrane surface (a micelle based on sodium dodecyl sulfate) was determined and described by $^1\mathrm H$ NMR spectroscopy and two-dimensional NMR (TOCSY, HSQC-HECADE, NOESY) spectroscopy. The complexation was confirmed by the change in the chemical shifts of the heptapeptide's $^1\mathrm H$ NMR spectra as well as by the signs and values of NOE effects in various media. A comparison of the spatial structure of heptapeptide Glu-Ile-Leu-Asn-His-Met-Lys in water solution and in the complex was made.

Détail

Keywords: oligopeptides, NMR $^1\mathrm H$ spectroscopy, TOCSY, NOESY.
Mots-clés : micelles

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