High resolution NMR in biofluids and tissues

U. Eichhoff   ; M. Spraul   ; M. Piotto

Učënye zapiski Kazanskogo universiteta. Seriâ Fiziko-matematičeskie nauki, Uchenye Zapiski Kazanskogo Universiteta. Seriya Fiziko-Matematicheskie Nauki, Tome 154 (2012) no. 1, pp. 23-32

Résumé

Bodyfluids are complicated mixtures and each component has its own NMR spectrum. The total spectrum may contain several thousand lines, but with the help of modern homo- and heteronuclear multidimensional NMR methods all lines can be assigned and a metabolite data base can be created. A principal component analysis of all spectra makes it possible to reveal deviation from the normal state. Spectra corresponding to this deviation then have to be evaluated more carefully using spectra simulation and data base programs. Tissues are heterogenous samples and in normal NMR spectra exhibit comparably broad lines due to a residual dipole coupling. Magic angle spinning (MAS) averages out these interactions leading to well resolved spectra which allow the identification of various metabolites. Metabolic changes due to malignant diseases can be detected, which is the basis of NMR-histology, a new discipline that has already been applied in various hospitals.

Détail

Keywords: NMR, magic angle spinning, NMR-histology, oncology, tissues, biofluids.

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