Geodesic
Interaction of $\mathrm{TOEPyP4}$ porphyrin with $\mathrm{A}$ form of DNA
Proceedings of the Yerevan State University. Physical and mathematical sciences, no. 3 (2014), pp. 43-48 Cet article a éte moissonné depuis la source Math-Net.Ru

Voir la notice de l'article

The peculiarities of binding the water-soluble meso-tetra-(4N-oxyethylpyridyl) porphyrin ($\mathrm{TOEPyP4}$) with $\mathrm{B}$-DNA and $\mathrm{A}$-DNA have been studied by means of UV-VIS spectrophotometry and circular dichroism method. The binding constant ($K_b$) and stoichiometry ($n$) were determined based on the absorbance spectra for each DNA–porphyrin complex. The free energy, enthalpy and entropy of binding also were calculated using the values of $K_b$. A comparative analysis with $\mathrm{TOEPyP4-B}$-DNA complex was performed. The obtained results show that the porphyrin interacts with $\mathrm{B}$-DNA by way of intercalation at low relative concentrations ($r = C_{porf}/C_{DNA}$) and external binding at large values of $r$, whereas the interaction of $\mathrm{TOEPyP4}$ with $\mathrm{A}$-DNA occurs via the outside binding mode only.
Keywords: porphyrin, thermodynamics, absorbance spectra, circular dichroism.
Mots-clés : DNA conformation 
@article{UZERU_2014_3_a7,
     author = {A. A. Avetisyan},
     title = {Interaction of $\mathrm{TOEPyP4}$ porphyrin with $\mathrm{A}$ form of {DNA}},
     journal = {Proceedings of the Yerevan State University. Physical and mathematical sciences},
     pages = {43--48},
     year = {2014},
     number = {3},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/UZERU_2014_3_a7/}
}
TY  - JOUR
AU  - A. A. Avetisyan
TI  - Interaction of $\mathrm{TOEPyP4}$ porphyrin with $\mathrm{A}$ form of DNA
JO  - Proceedings of the Yerevan State University. Physical and mathematical sciences
PY  - 2014
SP  - 43
EP  - 48
IS  - 3
UR  - http://geodesic.mathdoc.fr/item/UZERU_2014_3_a7/
LA  - en
ID  - UZERU_2014_3_a7
ER  - 
%0 Journal Article
%A A. A. Avetisyan
%T Interaction of $\mathrm{TOEPyP4}$ porphyrin with $\mathrm{A}$ form of DNA
%J Proceedings of the Yerevan State University. Physical and mathematical sciences
%D 2014
%P 43-48
%N 3
%U http://geodesic.mathdoc.fr/item/UZERU_2014_3_a7/
%G en
%F UZERU_2014_3_a7
A. A. Avetisyan. Interaction of $\mathrm{TOEPyP4}$ porphyrin with $\mathrm{A}$ form of DNA. Proceedings of the Yerevan State University. Physical and mathematical sciences, no. 3 (2014), pp. 43-48. http://geodesic.mathdoc.fr/item/UZERU_2014_3_a7/

[1] M.G. Vicente, “Porphyrin-Based Sensitizers in the Detection and Treatment of Cancer: Recent Progress”, Curr. Med. Chem. Anti-Cancer Agents, 1 (2001), 175–194 | DOI

[2] L.G. Marzilli, “Medical Aspects of DNA-Porphyrin Interactions”, New J. Chem., 14 (1990), 409–420

[3] R.F. Pasternack, E.J. Gibbs, Metal Ions in Biological Systems, eds. Sigel A., Sigel H., Marcel Dekker, NY, 1996, 367–397

[4] Y.-M. Chang, K.-M. Cammy, Ch. Hou, M.-H. Hou, “Conformational Changes in DNA Upon Ligands Binding Monitored by Circular Dichroism”, Int. J. Mol. Sci., 13 (2012), 3394–3413 | DOI

[5] E.J. Gibbs, R.F. Pasternack, “Interaction of Porphyrins and Metalloporphyrins with Nucleic Acids”, Semin Hematol, 26:2 (1989), 77–85

[6] R.F. Pasternack, “Circular Dichroism and the Interactions of Water Soluble Porphyrins with DNA”, Chirality, 15 (2003), 329–332 | DOI

[7] J.J. Correia, J.B. Chaires, “Analysis of drug-DNA Binding Isotherms: a Monte Carlo Approach”, Methods in Enzymology, 240 (1994), 593–614 | DOI

[8] V.I. Ivanov, L.E. Minchenkova, E.E. Minyat, M.D. Frank-Kamenetski, A.K. Schyolkina, “The $\mathrm{B}$ to $\mathrm{\bar{A}}$ Transition of DNA in Solution”, J. Mol. Biol., 87 (1974), 817–833 | DOI

[9] E.P. Geiduschek, I. Gray, J. Amer. Chem. Soc., 84 (1962), 3843–3852 | DOI

[10] A.F. Usatyi, L.S. Shlyakhtenko, “Melting of DNA in Ethanol–Water Solutions”, Biopolymers, 13 (1974), 2435–2446 | DOI

[11] T. Uno, K. Hamasaki, M. Tanigawai, S. Shimabayash, “Binding of Meso-tetrakis(Nmethylpyridinium-4-yl) porphyrin to Double Helical RNA and DNA RNA Hybrids”, Inorg. Chem., 36 (1997), 1676–1683 | DOI

[12] R.F. Pasternack, E.J. Gibbs, J.J. Villafranca, “Interactions of Porphyrins with Nucleic Acids”, Biochem., 22 (1983), 2406–2414 | DOI