Sodium-dependent amino acid transport in plasma membrane vesicles from rat liver

Leonardi, Maria Giovanna   ; Comolli, Roberto   ; Giordana, Barbara

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 80 (1986) no. 5, pp. 313-322

Résumé

Sono state preparate vescicole di membrana plasmatica da epatociti di ratto secondo due differenti metodiche, per saggiarne l'idoneità come substrato per lo studio del trasporto degli aminoacidi, tappa fondamentale del metabolismo aminoacidico nel fegato. A tale scopo sono stati studiati i sistemi di trasporto dell'alanina e dell'istidina. Si è potuto così identificare una preparazione in grado di conservare la capacità di accumulare entrambi gli aminoacidi con modalità e specificità, sia riguardo al substrato che agli ioni cotrasportati, analoghe a quelle già osservate nell'epatocita isolato.

Bibliographie

[1] J.D. Mcgivan, J.C. Ramsell and J.H. Lacey (1981) - Stimulation of alanine transport and metabolism by dibutyryl cyclic AMP in the hepatocytes from fed rats. «Biochim. Biophys. Acta», 644, 295-304.

[2] J.M.M. Van Amelsvoort, H.J. Sips and K. Van Dam (1978) - Sodium dependent alanine transport in plasma-membrane vesicles from rat. «Biochem. J.», 174, 1083-1086.

[3] N. Kraus-Friedmann, J. Biber, H. Murer and E. Carafoli (1982) - Calcium uptake in isolated plasma-membrane vesicles. «Eur. J. Biochem.», 129, 7-12.

[4] R.J. Epping and F.L. Bygrave (1984) - A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting $Ca^{2+}$-transport and $Ca^{2+}$-ATPase activities. «Biochem. J.», 223, 733-745.

[5] W. Schoner, C. Von Ilberg, R. Kramer and W. Seubert (1967) - On the mechanism of $Na^{+}$ and $K^{+}$-stimulated hydrolysis of adenosine triphosphate. «Eur. J. Biochem.», 1, 334-345.

[6] R.M. Michell and J.N. Hawthorne (1965) - The site of diphosphoinositine synthesis in rat liver. «Biochem. Biophys. Res. Commun.», 31, 333-338.

[7] L. Smith (1955) - Spectrophotometry assay of cytochrome c oxidase. In: «Methods of Biochemical analysis», vol. 2, Glick D. Ed., pp. 427-437. Interscience Pub., New York.

[8] B.S.S. Masters, C.H. Williams Jr. and H. Kamin (1967) - The preparation and properties of microsomal TPNH-cytochrome c reductase from pig liver. «Methods in Enzymology», X, 565-573.

[9] M.M. Bradford (1976) - A rapid and sensitive method for the quantitation of protein utilizing the principle of dye-protein binding. «Anal. Biochem.», 72, 248-254.

[10] H. Kessler and G. Semenza (1983) - The small-intestinal $Na^{+}$, D-glucose cotransporter: an asymmetric gate channel (or pore) responsive to $\nabla\Psi$. «J. Membrane Biol.», 76, 27-56.

[11] H.J. Sips, J.M.M. Van Amelsvoort and K. Van Dam (1980) - Amino acid transport in plasma membrane vesicles from rat liver. «Eur. J. Biochem.», 105, 216-224.

[12] M. Samson and M. Fehlmann (1982) - Plasma membrane vesicles from isolated hepatocytes retain the increase of amino acid transport by dibutyryl cyclic AMP in intact cells. «Biochim. Biophys. Acta», 687, 35-41.

[13] E. Heinz and A.M. Weinstein (1984) - The overshoot phenomenon in cotransport. «Biochim. Biophys. Acta», 776, 83-91.

[14] L.O. Kristensen (1980) - Energization of alanine transport in isolated rat hepatocytes. «J. Biol. Chem.», 255, 5236-5243.

[15] M.S. Kilberg (1982) - Amino acid transport in isolated rat hepatocytes. «J. Membrane Biol.», 69, 1-12.

[16] M.S. Kilberg, H.E. Handlogten and H.N. Christensen (1980) - Characteristics of an amino acid transport system in rat liver for glutamine, asparagine, histidine and closely related analogs. «J. Biol. Chem.», 255, 4011-4019.

[17] J.W. Edmonson, L. Lumeng and T.-K. Li (1979) - Comparative studies of alanine and $\alpha$-aminoisobutyric acid uptake by freshly isolated rat liver cells. «J. Biol. Chem.», 254, 1653-1658.

[18] M.S. Kilberg, M.E. Hanlogten and H.N. Christensen (1981) - Characteristics of system ASC for transport of neutral amino acids in isolated rat hepatocyte. «J. Biol. Chem.», 256, 3304-3312.

[19] D.C. Quinlan, C.G. Todderud, D.S. Kelley and R.F. Kletzien (1982) - Sodium-gradient-stimulated transport of L-alanine by plasma-membrane vesicles isolated from liver parenchymal cells of fed and starved rats. «Biochem. J.», 208, 685-693.

[20] J.W. Edmondson, L. Lumeng and T.-K. Li (1977) - Direct measurement of active transport systems for alanine in freshly isolated rat liver cells. «Biochem. Biophys. Res. Commun.», 76, 751-757.

[21] A. Le Cam and P. Freychet (1977) - Neutral amino acid transport characterization of the A and L systems in isolated rat hepatocytes. «J. Biol. Chem.», 252, 148-156.

[22] M. Fehlmann, A. Le Cam, P. Kitabgi, J.-F. Rey and P. Freychet (1979) - Regulation of amino acid transport in the liver. Emergence of a high affinity transport system in isolated hepatocytes from fasting rats. «J. Biol. Chem.», 254, 401-407.

[23] R.J. Dorio, J. Hock and E. Rubin (1984) - Ethanol treatment selectively decreases neutral amino acid transport in cultured hepatocytes. «J. Biol. Chem.», 259, 11430-11435.