Sodium-dependent amino acid transport in plasma membrane vesicles from rat liver

Leonardi, Maria Giovanna ; Comolli, Roberto ; Giordana, Barbara

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 80 (1986) no. 5, pp. 313-322.

Résumé

Sono state preparate vescicole di membrana plasmatica da epatociti di ratto secondo due differenti metodiche, per saggiarne l'idoneità come substrato per lo studio del trasporto degli aminoacidi, tappa fondamentale del metabolismo aminoacidico nel fegato. A tale scopo sono stati studiati i sistemi di trasporto dell'alanina e dell'istidina. Si è potuto così identificare una preparazione in grado di conservare la capacità di accumulare entrambi gli aminoacidi con modalità e specificità, sia riguardo al substrato che agli ioni cotrasportati, analoghe a quelle già osservate nell'epatocita isolato.

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