Geodesic
Preparation of isolated brush border membranes from rabbit gallbladder epithelium: a preliminary study on the presence of the anion sensitive ATPase activity
Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 73 (1982) no. 5, pp. 162-174 Cet article a éte moissonné depuis la source Biblioteca Digitale Italiana di Matematica

Voir la notice de l'article

Nelle cellule epiteliali di cistifellea di coniglio sono state saggiate l'attività della ATPasi $Na^{+}—K^{+}$, della ATPasi anionica, della saccarasi, leucinoaminopeptidasi e fosfatasi alcalina. Si è tentato inoltre l’isolamento dell’intero orletto a spazzola e di frammenti di membrane apicali, nelle quali si è ricercata la presenza dell’ATPasi anionica (che potrebbe essere alla base dei trasporti di $HCO^{-}_{3}$, $Cl^{—}$ e $H^{+}$ rilevati in cistifellea). Come risultato l’ATPasi $Na^{+}—K^{+}$ è stata proposta come marcatore delle membrane baso-laterali e la fosfatasi alcalina delle membrane plasmatiche soprattutto apicali di cistifellea. Non si è riusciti ad isolare l’orletto a spazzola intero, ma si è giunti ad un discreto isolamento di membrane plasmatiche apicali col metodo della precipitazione con $Ca^{2+}$. Non è stata rilevata alcuna attività dell’ATPasi-anionica di tipo mitocondriale in questo tipo di membrane. 
@article{RLINA_1982_8_73_5_a11,
     author = {Cremaschi, Dario and Meyer, Giuliano and Bermano, Sandra},
     title = {Preparation of isolated brush border membranes from rabbit gallbladder epithelium: a preliminary study on the presence of the anion sensitive {ATPase} activity},
     journal = {Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali},
     pages = {162--174},
     year = {1982},
     volume = {Ser. 8, 73},
     number = {5},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/RLINA_1982_8_73_5_a11/}
}
TY  - JOUR
AU  - Cremaschi, Dario
AU  - Meyer, Giuliano
AU  - Bermano, Sandra
TI  - Preparation of isolated brush border membranes from rabbit gallbladder epithelium: a preliminary study on the presence of the anion sensitive ATPase activity
JO  - Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali
PY  - 1982
SP  - 162
EP  - 174
VL  - 73
IS  - 5
UR  - http://geodesic.mathdoc.fr/item/RLINA_1982_8_73_5_a11/
LA  - en
ID  - RLINA_1982_8_73_5_a11
ER  - 
%0 Journal Article
%A Cremaschi, Dario
%A Meyer, Giuliano
%A Bermano, Sandra
%T Preparation of isolated brush border membranes from rabbit gallbladder epithelium: a preliminary study on the presence of the anion sensitive ATPase activity
%J Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali
%D 1982
%P 162-174
%V 73
%N 5
%U http://geodesic.mathdoc.fr/item/RLINA_1982_8_73_5_a11/
%G en
%F RLINA_1982_8_73_5_a11
Cremaschi, Dario; Meyer, Giuliano; Bermano, Sandra. Preparation of isolated brush border membranes from rabbit gallbladder epithelium: a preliminary study on the presence of the anion sensitive ATPase activity. Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 73 (1982) no. 5, pp. 162-174. http://geodesic.mathdoc.fr/item/RLINA_1982_8_73_5_a11/

[1] Coleman R. and Finean J.B. (1966) — Preparation and properties of isolated plasma membranes from guinea-pig tissues. «Biochim. Biophys. Acta», 125, 197-206.

[2] Cremaschi D. and Henin S. (1975) — Extracellular space determination in gallbladder mucosa. «Biochim. Biophys. Acta», 411, 291-294.

[3] Cremaschi D., Henin S. and Meyer G. (1979) - Stimulation by $HCO_{3}^{-}$ of $Na^{+}$ transport in rabbit gallbladder. «J. Membrane Biol.», 47, 145-170.

[4] De Pont J.J.H.H.M., Hansen T. and Bonting S.L. (1972) - An anion sensitive ATPase in lizard gastric mucosa. «Biochim. Biophys. Acta», 274, 189-200.

[5] De Renzis G. and Bornancin M. (1977) - A $Cl^{-}/HCO_{3}^{-}$-ATPase in the gills of Carassius auratus. Its inhibition by thiocyanate. «Biochim. Biophys. Acta», 467, 192-207.

[6] Diamond J.M. (1964) - Transport of salt and water in rabbit and guinea-pig gallbladder. «J. Gen. Physiol.», 48, 1-14.

[7] Ebel R.E. and Lardy H.A. (1975) - Stimulation of rat liver mitochondrial adenosine triphosphatase by anions. «J. Biol. Chem.», 250, 191-196.

[8] Eichholz A. (1967) - Structural and functional organization of the brush border of intestinal epithelial cells. III. Enzymic activities and chemical composition of various fractions of tris-disrupted brush-borders. «Biochim. Biophys. Acta», 135, 475-482.

[9] Fanestil D.D., Hastings A.B. and Mohawald T.A. (1963) - Environmental $CO_{2}$ stimulation of mitochondrial adenosine triphosphatase activity. «J. Biol. Chem.», 238, 836-842.

[10] Forstner G.G., Sabesin S.M. and Issflbacher K.J. (1968) - Rat intestinal microvillus membranes. Purification and biochemical characterization. «Biochem. J.», 106, 381-390.

[11] Hausamen T.U., Helger R., Rick W. and Groos W. (1967) - Optimal conditions for the determination of serum alkaline phosphatase by a new kinetic method. «Clin. Chim. Acta», 15, 241-245.

[12] Henin S., Cremaschi D., Schettino T., Meyer G., Lora Lamia Donin C. and Cotelli F. (1977) - Electrical parameters in gallbladders of different species. Their contribution to the origin of the transmural potential difference. «J. Membrane Biol.», 34, 73-91.

[13] Humphreys M.H. and Chou L.Y.N. (1979) - Anion stimulated ATPase activity of brush border from rat small intestine. «Am. J. Physiol.», 236, E70—E76.

[14] Izutsu K.I. and Siegel I.A. (1972) - A microsomal $HCO_{2}^{-}$-stimulated ATPase from the dog submandibular gland. «Biochim. Biophys. Acta», 284, 478-484.

[15] Kaye G.I., Wheeler H.O., Whitlock R.T. and Lane N. (1966) - Fluid transport in the rabbit gallbladder. A combined physiological and electromicroscopic study. «J. Cell. Biol.», 30, 237-268.

[16] Kasbekar D.K. and Durbin R.P. (1965) - An adenosine triphosphatase from frog gastric mucosa. «Biochim. Biophys. Acta», 105, 472-482.

[17] Kessler M., Acuto O., Storelli C., Murer H., Muller M. and Semenza G. (1978) - A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and choline transport systems. «Biochim. Biophys. Acta», 506, 136-154.

[18] Kimelberg H.K. and Bourke R.S. (1972) - A bicarbonate-stimulated, thiocyanate-inhibited ATPase in mammalian brain and kidney. «Biphys. J.», 12, 191a.

[19] Kinne-Saffran E. and Kinne R. (1974) - Presence of a bicarbonate-stimulated ATPase in the brush border microvillus membranes of the proximal tubule. «Proc. Soc. Exp. Biol. Med.», 146, 751-753.

[20] Kinne-Saffran E. and Kinne R. (1979) - Further evidence for the existence of an intrinsic bicarbonate-stimulation $Mg^{2+}$-ATPase in brush border membranes isolated from rat kidney cortex. «J. Membrane Biol.», 49, 235-251.

[21] Lambeth D.O. and Lardy H.A. (1971) - Purification and properties of rat liver mitochondrial adenosinetriphosphatase. «Eur. J. Biochem.», 22, 355—363. | MR

[22] Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951) - Protein measurement with the Folin phenol reagent. «J. Biol. Chem.», 193, 265—275.

[23] Malathi P., Preiser H., Fairclough P., Mallet P. and Crane R.K. (1979) - A rapid method for the isolation of kidney brush border membranes. «Biochim. Biophys. Acta», 554, 259—263.

[24] Miller D. and Crane R.K. (1961) - The digestive function of the epithelium of the small intestine. II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells. «Biochim. Biophys. Acta», 52, 293-298.

[25] Mills J.W. and Di Bona D.R. (1978) - Distribution of $Na^{+}$ pump sites in the frog gallbladder. «Nature», Lond. 271, 273-275.

[26] Mircheff A.K. and Wright E.M. (1976) — Analytical isolation of plasma membranes of intestinal epithelial cells. Identification of $Na^{+}—K^{+}$-ATPase rich membranes and the distribution of enzyme activities. «J. Membrane Biol.», 28, 309-333.

[27] Nagel W., Willig F. and Schmidt F.H. (1964) - Über die aminosaureärylamidase- (sog. Leucinaminopeptidase)-aktivität im menschlichen serum. «Klin. Wschr.», 42, 447-449.

[28] Petersen K.U. and Heintze K. (1982) - The double ion exchange model of $NaCl$ influx into gallbladder cells: butyrate uptake rates. In: «Electrolyte and water transport across gastrointestinal epithelia»; ed. Case R.M. et al., Raven Press, N. York, 209-214.

[29] Sachs G., Shah G., Strych A., Cline G. and Hirschowitz B.I. (1972) - Properties of ATPases of gastric mucosa. III. Distribution of $HCO_{3}^{-}$-stimulated APTase in gastric mucosa. «Biochim Biophys. Acta», 266, 625-638.

[30] Schmitz J., Preiser H., Maestracci D., Ghosch B.K., Cerda J.J. and Crane R.K. (1973) - Purification of the human intestinal brush border membrane. «Biochim. Biophys. Acta», 323, 98-112.

[31] Simon B., Kinne R. and Sachs G. (1972) - The presence of $HCO_{3}^{-}$-ATPase in pancreatic tissue. «Biochim. Biophys. Acta», 282, 293-300. | MR

[32] Simon B., Kinne R. and Knauf H. (1972) - The presence of $HCO_{3}^{-}$-ATPase in glandula submandibularis of rabbit. «Pflügers Arch.», 337, 177—184. | MR

[33] Simon B. and Thomas L. (1972) - $HCO_{3}^{-}$ stimulated ATPase from mammalian pancreas, properties and its arrangement with other enzyme activities. «Biochim. Biophys. Acta», 288, 434-442.

[34] Smith L. (1955) - Spectrophotometric assay of cytochrome c oxidase. In: «Methods of Biochemical analysis» ed. Glick, D., Interscience Publ. Inc., New York 2, 427-434.

[35] Soumarmon A., Lewin M., Cheret A. and Bonfils S. (1974) - Gastric $HCO_{3}^{-}$ stimulated ATPase. Evidence against its microsomal localization in rat fundus mucosa. «Biochim. Biophys. Acta», 339, 403-414.

[36] Thomas L. and Kinne R. (1972) - Studies on the arrangement of the aminopeptidase and alkaline phosphatase in the microvilli of isolated brush border of rat kidney. «Biochim. Biophys. Acta», 255, 114—125.

[37] Van Amelsvoort J.M.M., De Pont J.J.H.H.M. and Bonting S.L. (1977) - Is there a plasma membrane-located anion-sensitive ATPase? «Biochim. Biophys. Acta», 466, 283—301.

[38] Van Amelsvoort J.M.M., De Pont J.J.H.H.M., Stols A.L.H. and Bonting S.L. (1977) — Is there a plasma membrane-located anion-sensitive ATPase? Further studies on rabbit kidney. «Biochim. Biophys. Acta», 471, 78—91.

[39] Van Os C.H., Mircheff A.K. and Wright E.M. (1977) - Distribution of bicarbonate stimulated ATPase in rat intestinal epithelium. «J. Cell. Biol.» 73, 257-260.

[40] Van Os C.H. and Slegers J.F.G. (1970) - Characteristics of $Na^{+}-K^{+}$ stimulated ATPase in rabbit gallbladder epithelium. «Pflügers Arch.», 319, 49-56.

[41] Whitloch R.T. and Wheeler H.O. (1969) - Hydrogen ion transport by isolated rabbit gallbladder. «Am. J. Physiol.», 217, 310-316.

[42] Wiebelhaus V.D., Sung C.P., Helander H.F., Shah G., Blum A.L. and Sachs G. (1971) - Solubilization of anion ATPase from Necturus oxyntic cells. «Biochim. Biophys. Acta», 241, 49-56.

[43] Wright E.M. and Diamond J.M. (1969) — An electrical method of measuring nonelectrolyte permeability. «Proc. Roy. Soc.», B 172, 203-225.

[44] Zilversmit D.B. and Davis A.K. (1950) - Microdetermination of plasma phospholipids by trichloracetic acid precipitation. «J. Lab. Clin. Med.», 35, 155—160.