I cariotipi di Triturus marmoratus e T. cristatus studiati con il "C-staining method"

Mancino, Giorgio ; Ragghianti, Matilde ; Bucci Innocenti, Stefania

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 55 (1973) no. 5, pp. 559-564.

Résumé

The mitotic chromosomes of the newt species Triturus marmoratus and T. cristatus were submitted to the C -staining method. All the centromeres are darkly stained and single darkly stained bands are induced on both sides of the centromere regions as well as on various chromosomes in subterminal position. Therefore, the C-bands allow the identification of the single elements of the complements and their more exact arrangement into karyotypes. In both species, the sex chromosomes show an extensive dark segment along the long arm corresponding to the heteromorphie region of lampbrush chromosomes I. In spite of the different number and location of the nucleolus-organizing regions and some discrepancies concerning certain subterminal bands, the two karyotypes appear of similar morphology and structure. There is no evidence for extensive structural rearrangements: probably T. marmoratus and T. cristatus have mostly undergone a certain differentiation caused by allelic changes in single genetic loci during the processes of karyological evolution and speciation.

Bibliographie

Arrighi F. E. e Hsu T. C., Localization of heterochromatin in human chromosomes, «Cytogenetics», 10, 81-86 (1971).

Callan H. G. e Lloyd L., Lampbrush chromosomes of crested newts Triturus cristatus (Laurenti), «Phil. Trans. Lond.», B 243, 135-219 (1960 a).

Callan H. G. e Lloyd L., Lampbrush chromosomes. In: «New approaches in cell biology» (Walker, P.M.B. ed.). New York: Academic Press (1960 b).

Cooper J. E. K. e Hsu T. C., The C-band and G-band patterns of Microtus agrestis chromosomes, «Cytogenetics», 11, 295-304 (1972).

Gall J. G. e Pardue M. L., Nucleic acid hybridization in cytological preparation, «Methods in enzymology», 31, 470-480. New York: Academic Press (1971). (Grossman L. and Moldave K., eds.).

Ganner E. e Evans H. J., The relationship between patterns of DNA replication and of quinacrine fluorescence in the human chromosome complement, «Chromosoma», 35, 326-341 (1971).

Labrousse M., Guillemin C. e Gallien L., Mise en évidence, sur les chromosomes de L'Amphibien Pleurodeles waltlii Michah., de secteurs d'affinité différente pour le colorant de Giemsa à pH 9, «C.R. Acad. Sc. Paris», 274, 1063-1065 (1972).

Lantz L. A., Hybrids between Triturus cristatus Laur. and Triturus marmoratus Latr., «Proc. Zool. Soc.», 117, 247-258 (1947).

Levan A., Fredga K. e Sandberg A. A., Suggestion for a chromosome nomenclature based on centromeric position, «Hereditas», 52, 201-220 (1964).

Mancino G., Lampbrush chromosomes in cytotaxonomic and cytogenetic studies. In: «Cytotaxonomy and Vertebrate Evolution» (Chiarelli B. and Capanna E., eds.). New York: Academic Press (1973).

Mancino G. e Nardi I., Chromosomal heteromorphism and female heterogamety in the marbled newt Triturus marmoratus (Latreille), «Experientia», 27, 821-822 (1971).

Mancino G., Nardi I. e Ragghianti M., Lampbrush chromosomesfrom semi-albino crested newts, Triturus cristatus carnifex, «Experientia», 28, 856-860 (1972).

Nardi I. e Mancino G., Mitotic karyotype and nucleoli of the marbled newt Triturus marmoratus (Latreille), «Experientia», 27, 424-425 (1971).

Nardi L., Ragghianti M. e Mancino G., Karyological differentiation within the superspecies Triturus cristatus (Amphibia, Urodela), «Boll. Zool.», 37, 511-512 (1970).

Nardi L., Ragghianti M. e Mancino G., Characterization of the lampbrush chromosomes of the marbled newt Triturus marmoratus (Latreille, 1800), «Chromosoma», 37, 1-22, (1972).

Nardi L., Ragghianti M. e Mancino G., Banding patterns in newt chromosomes by the Giemsa stain, «Chromosoma», 40, 321-331 (1973).

Pardue M. L. e Gall J. G., Chromosomal localization of mouse satellite DNA, «Science», 168, 1356-1358 (1970).

Paris Conference (1971), Standardization in human cytogenetics, «Cytogenetics», 11, 313-362 (1972).

Spurway H., Genetics of specific and subspecific differences in European newts, «Symp. Soc. Explt. Biol.», 7, 200-237 (1953).

Thorn R., Les Salamandres, Éditions P. Lechevalier, Paris (1968).

Vallée L., Recherches sur Triturus blasii de l'Isle, hybrid naturel de Triturus cristatus Laur. x Triturus marmoratus Latr., Imprimerie M. Declume, Lons-le-Saunier (1959).

Vosa C. G. e Marchi P., On the quinacrine fluorescence and Giemsa staining patterns of the chromosomes of Vicia faba, «Giorn. Bot. It.», 106, 151-159 (1972).

White M. J. D., Chromosomal rearrangements and speciation in animals, «Annual Review of Genetics» (Roman H. L. ed.), 3, 75-98 (1969).

Yunis J. J. e Yasmineh W. G., Heterochromatin, satellite DNA, and cell function, «Science», 174, 1200-1209 (1971).