Osservazioni istochimiche sulla $\beta$-glucuronidasi nel mesonefro in regressione dell'embrione di pollo

Russo-Caia, Salvatore   ; Palatroni, Pietro

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 51 (1971) no. 5, pp. 434-439

Résumé

Histochemical observations have been made by the Hayashi simultaneous coupling method (naphtol AS-BI glucuronide-hexazonium pararosanilin) on the localization of $\beta$-glucuronidase in the growing and regressing chick embryo mesonephros. The observed aspects of cellular degeneration are the formation of cytolysomes and of masses due to their aggregation; in more advanced stages, and particularly in the residual tubules which persist even after hatching, the enzyme is localized in still larger masses; in the connective tissue the reaction is strongly positive in the phagocytic cells. These aspects, similar to those previously observed for the acid phosphatase, demonstrate the direct intervention of lysosomal $\beta$-glucuronidase in the auto and heterolytic processes which occur in the involution of the intermediate kidney. Considering the dual localization of the enzyme (in lysosomes and in endoplasmic reticulum), we are now working on the same material-with different histochemical methods-to elucidate the relationship between the two different activities in the growth and regression.

Bibliographie

Barrett A. J., Properties of lysosomal enzymes, in «Lysosomes in Biology and Pathology» (Eds. J. T. Dingle & H. B. Fell), 2, cap. 10; North-Holland, Amsterdam (1969).

Beck F. e Lloyd J. B., Histochemistry and electron microscopy of lysosomes, in «Lysosomes in Biology and Pathology» (Eds. J. T. Dingle & H. B. Fell), 2, cap. 21; North-Holland, Amsterdam (1969).

De Duve C., The lysosome in retrospect, in «Lysosomes in Biology and Pathology» (Eds. J. T. Dingle & H. B. Fell), I, cap. 1; North-Holland, Amsterdam (1969).

Fishman W. H., Goldman S. S. e Delellis R., Dual localization of $\beta$-glucuronidase in endoplasmic reticulum and in lysosomes, «Nature», 213, 457 (1967).

Fishman W. H., Ide H. e Rufo R., Dual localisation of acid hydrolases in endoplasmic reticulum and in lysosomes. I. $\beta$-glucuronidase staining reactions and cytochemical studies on kidney in androgen-stimulated mice, «Histochemie», 20, 287 (1969 a).

Ganschow R. e Paigen K., Separate genes determining the structure and intracellular location of hepatic glucuronidase, «Proc. Natl. Acad. Sci.», 58, 938 (1967).

Hayashi M., Distribution of $\beta$-glucuronidase activity in rat tissues employing the naphtol AS-BI glucuronide hexazonium pararosanilin method, «J. Histoch. & Cytoch.», 12, 659 (1964).

Hayashi M., Nakajima Y. e Fishman W. H., The cytologic demonstration of $\beta$-glucuronidase employing naphtol AS-BI glucuronide and hexazonium pararosanilin; a preliminary report, «J. Histoch. & Cytoch.», 12, 293 (1964).

Hayashi M., Shirahama T. e Cohen A. S., Combined cytochemical and electron microscopic demonstration of $\beta$-glucuronidase activity in rat liver with the use of a simultaneous coupling azo dye technique, «J. Cell Biol.», 36, 289 (1968).

Ide H. e Fishman W. H., Dual localisation of $\beta$-glucuronidase and acid phosphatase in lysosomes and in microsomes. II. Membrane—associated enzymes, «Histochemie», 20, 300 (1969 b).

Maunsbach A. B., Functions of lysosomes in kidney cells, in «Lysosomes in Biology and Pathology» (Eds. J. T. Dingle & H. B. Fell), 1, cap. 4; North-Holland, Amsterdam (1969).

Patel V. B., Some aspects of the hydrolysis of phosphomonoesters by homogenates of growing and regressing mesonephroi in chick embryos, «Experientia», 23, 801 (1967).

Patel V. B., Acid hydrolases and their relationship to lysosomes in the mesonephros of the chick embryo, «J. Exp. Zool.», 175, 429 (1970).

Price R. G. e Dance N., The cellular distribution of some rat-kidney glycosidases, «Biochem. J.», 105, 877 (1967).

Russo-Caia S., Attività enzimatiche nel meso e metanefro dell'embrione di pollo, «Boll. Zool.», 31, 875 (1964).

Russo-Caia S., Studi sul mesonefro e metanefro dell'embrione di pollo, «Acta Med. Rom.», 4, 469 (1966).

Russo-Caia S. e Hassan G., Attività solubile e sedimentabile delle fosfatasi acide nello sviluppo e nella regressione del mesonefro dell'embrione di pollo, «Acta Embryol. Morphol. Exp.», 8, 119 (1965 a).

Russo-Caia S. e Hassan G., Osservazioni istochimiche sulla glicerofosfatasi acida nel mesonefro in regressione dell'embrione di pollo, «Rend. Acc. Naz. Lincei», 39, 563 (1965 b).

Russo-Caia S. e Cecere F., Solubilizzazione di enzimi lisosomiali (proteinasi e ribonucleasi acide) durante la regressione del mesonefro dell'embrione di pollo, «Ric. Scient.», 36, 1047 (1966).

Salzgeber B. e Weber R., La régression du mésonéphros chez l'embryon de poulet. Étude des activités de la phosphatase acide et des cathepsines: analyse biochimique, histochimique et observations au microscope électronique, «J. Embryol. Exp. Morphol.», 15, 397 (1966).

Stampfli H. R., Histologische Studien am Wolff'sehen Körper (Mesonephros) der Vögel und über seinen Umbau zu Nebenhoden und Nebenovar, «Rev. Suisse Zool.», 57, 237 (1950).

Straus W., Lysosomes, phagosomes and related particles, in «Enzyme Cytology» (Ed. D. B. Roodyn), cap. 5; Acad. Press, N.Y. (1967).

Tappel A. L., Lysosomal enzymes and other components, in «Lysosomes in Biology and Pathology» (Eds. J. T. Dingle & H. B. Fell), 2, cap. 9; North-Holland, Amsterdam (1969).