I precursori non fosforilati degli acidi nucleici nei cotiledoni germinanti di Phaseolus vulgaris

Bellando, Mario ; Fiussello, Nevio

Atti della Accademia nazionale dei Lincei. Rendiconti della Classe di scienze fisiche, matematiche e naturali, Série 8, Tome 41 (1966) no. 5, pp. 365-367

Résumé

The Authors have isolated and identified the purine and pyrimidine bases and related nucleosides occurring in the free state in cotyledons of etiolated dwarf bean seedling. 180 cotyledons from young etiolated plants (23-26 cm in length) were collected, washed and homogenized at 4°C. The resulting slurry was dyalized against fresh water and the proteins and polysaccharides-free solution concentrated and analyzed in an ion-exchange column (Dowex 2 X, formate). The elution was achieved with ammonium formate solutions with discontinuously increasing ionic strength and pH. The fractions were collected, dried in vacuo and the solid ammonium formate sublimed. The costiti!ents were purified chromatographically and identified by their U.V. absorbtion spectra at various pH. The relative abundance of the components was also, when possible, roughly evaluated.