Geodesic
Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis
Žurnal Sibirskogo federalʹnogo universiteta. Matematika i fizika, Tome 17 (2024) no. 6, pp. 791-797

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We employed DNA and 2'-fluoro RNA aptamers to assembly novel systems for colorimetric microplate detection of TNFa, interleukin-17A and Dickkopf-1 proteins as biomarkers associated with ankylosing spondylitis. For each protein, we chose an optimal sandwich pair made of plate-immobilized capture aptamer and biotinylated reporter aptamer recruiting streptavidin-peroxidase conjugate. A feasibility of these test systems was demonstrated in model protein solutions.
Keywords: oligonucleotide aptamers, biosensors, colorimetric detection, ankylosing spondylitis. 
@article{JSFU_2024_17_6_a11,
     author = {Elizaveta A. Shatunova and Anna S. Davydova and Nikolay A. Danilin and Arseniy A. Golyshkin and Maksim A. Korolev and Mariya A. Vorobyeva},
     title = {Design of aptamer sandwich pairs for colorimetric detection of {TNF{\cyra},} {Interleukin-17a} and {Dickkopf-1} proteins associated with ankylosing spondylitis},
     journal = {\v{Z}urnal Sibirskogo federalʹnogo universiteta. Matematika i fizika},
     pages = {791--797},
     publisher = {mathdoc},
     volume = {17},
     number = {6},
     year = {2024},
     language = {en},
     url = {http://geodesic.mathdoc.fr/item/JSFU_2024_17_6_a11/}
}
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%A Elizaveta A. Shatunova
%A Anna S. Davydova
%A Nikolay A. Danilin
%A Arseniy A. Golyshkin
%A Maksim A. Korolev
%A Mariya A. Vorobyeva
%T Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis
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Elizaveta A. Shatunova; Anna S. Davydova; Nikolay A. Danilin; Arseniy A. Golyshkin; Maksim A. Korolev; Mariya A. Vorobyeva. Design of aptamer sandwich pairs for colorimetric detection of TNFа, Interleukin-17a and Dickkopf-1 proteins associated with ankylosing spondylitis. Žurnal Sibirskogo federalʹnogo universiteta. Matematika i fizika, Tome 17 (2024) no. 6, pp. 791-797. http://geodesic.mathdoc.fr/item/JSFU_2024_17_6_a11/